anti stat3α  (Cell Signaling Technology Inc)

Journal: Journal of Ginseng Research

Article Title: Compound K-enriched Korean red ginseng prevents lung cancer progression by targeting cancer cells and fibroblasts

doi: 10.1016/j.jgr.2025.03.010

Figure Lengend Snippet: Pro-apoptotic effects of CKP in lung cancer cells (A) PARP, caspase-3, and survivin were detected by Western blot after applying CKP to A549 or H1299 cells for 30 h. GAPDH was used as the control. (B) Apoptotic cell death was determined by flow cytometry by Annexin V/PI staining in A549 and H1299 cells treated with CKP for 30 h (Left). A graph of increase in early and late apoptosis (Right). (C) Western blot of DR5, pSTAT3, STAT3α, pp38 and p38 in A549 and H1299 cells treated with CKP for 30 h. Results are presented as mean ± SD. ∗ p < 0.05, and ∗∗ p < 0.01 compared with the indicated control groups.

Article Snippet: Antibodies against the following proteins were used: STAT3α (Cell Signaling, #8768), phospho-specific STAT3 (Tyr 705) (Cell Signaling, #9131), DR5 (Cell Signaling, #3696), PARP (Cell Signaling, #9542, Danvers, MA, USA), Survivin (Cell Signaling, #2808), caspase-3 (Cell Signaling, #9662), p38 (Cell Signaling, #9212), phospho p38 (Thr180/Tyr182) (Cell Signaling, #4511), α-SMA (Sigma-Aldrich, A2547), collagen1α1 (Cell Signaling, #72026), fibronectin (R&D Systems, MAB 1918), E-cadherin (Cell Signaling, #4065), phospho-Smad2 (Ser465/467) (Cell Signaling, #3108) and GAPDH (Merck, CB1001, Darmstadt, Germany).

Techniques: Western Blot, Control, Flow Cytometry, Staining

Journal: Journal of Ginseng Research

Article Title: Compound K-enriched Korean red ginseng prevents lung cancer progression by targeting cancer cells and fibroblasts

doi: 10.1016/j.jgr.2025.03.010

Figure Lengend Snippet: Inhibition of EMT cha nge by CKP (A) A549 and WI-38 cells were co-cultured, and pSTAT3 (Y 705), STAT3α, p-Smad2 (Ser465/467), and E-cadherin levels were assessed by Western blot (Left). WI-38 cells induced A549-GFP cells wound healing was assessed by measuring migration distances of GFP-expressing cells over 24 h (Right). (B) The effect of galunisertib on the inhibition of wound healing by CKP in A549-GFP cells co-cultured with WI-38 cells. (C) The EMT changes induced by TGF-β1 in A549 cells were analyzed by Real-time PCR. (D) The effect of CKP on wound healing by TGF-β1 treated A549 cells. (E, F) The effect of CKP on the invasiveness of A549 treated with TGF-β1 were tested by transwell invasion assay. (G) Inhibition of A549-GFP cells wound healing by galunisertib (2 μM) in the presence of WI-38 cells and CKP. (H) Western blot of p-Smad2 in A549 cells co-cultured with WI-38 cells in the presence or absence of galunisertib and CKP. The red line represents the wound scratch, while the yellow line marks the wound 24 h post-injury. Values presented are means ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.005 compared with the indicated control groups.

Article Snippet: Antibodies against the following proteins were used: STAT3α (Cell Signaling, #8768), phospho-specific STAT3 (Tyr 705) (Cell Signaling, #9131), DR5 (Cell Signaling, #3696), PARP (Cell Signaling, #9542, Danvers, MA, USA), Survivin (Cell Signaling, #2808), caspase-3 (Cell Signaling, #9662), p38 (Cell Signaling, #9212), phospho p38 (Thr180/Tyr182) (Cell Signaling, #4511), α-SMA (Sigma-Aldrich, A2547), collagen1α1 (Cell Signaling, #72026), fibronectin (R&D Systems, MAB 1918), E-cadherin (Cell Signaling, #4065), phospho-Smad2 (Ser465/467) (Cell Signaling, #3108) and GAPDH (Merck, CB1001, Darmstadt, Germany).

Techniques: Inhibition, Cell Culture, Western Blot, Migration, Expressing, Real-time Polymerase Chain Reaction, Transwell Invasion Assay, Control

Journal: FEBS Open Bio

Article Title: The EGF / EGFR axis and its downstream signaling pathways regulate the motility and proliferation of cultured oral keratinocytes

doi: 10.1002/2211-5463.13653

Figure Lengend Snippet: List of reagents and antibodies used in this study.

Article Snippet: Stat3α (D1A5) XP ® Rabbit mAb , Cell Signaling Technology , #8768 , 1 : 1000.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Co-Expression of Podoplanin and CD44 in Proliferative Vitreoretinopathy Epiretinal Membranes

doi: 10.3390/ijms24119728

Figure Lengend Snippet: ( a ) Real-time qPCR on nine patients (x axis) revealed HMGB1 expression in all ERMs analyzed. Values are expressed as fold changes with respect to one arbitrarily chosen ERM specimen that was used as a reference sample for all the reactions (patient 1). ( b ) Immunofluorescence on ERMs with SOX2, STAT3, and YAP1 antibodies showing cytoplasmic and faint nuclear staining (arrows). Scale bar 10 μm.

Article Snippet: For protein immunofluorescent localization by confocal microscopy, the following primary antibodies were used: mouse anti-podoplanin monoclonal antibody (clone D2-40) from Dako (Glostrup, Denmark); rabbit anti-CD44 and anti-SOX2 polyclonal antibodies from Sigma (St. Louis, MO, USA); goat anti-vimentin polyclonal antibody [ ] kindly gifted from Prof. Peter Traub (Max-Planck-Institut fur Zellbiologie; Rosenhof, Ladenburg, Germany); polyclonal rabbit anti-GFAP from Zymed Laboratories (San Francisco, CA, USA); rabbit anti-pan cytokeratin polyclonal antibody from Bioss Antibodies (Woburn, MA, USA); and rabbit anti β-catenin monoclonal antibody (clone D10A8) was purchased from Cell Signaling Technology (Danvers, MA, USA), whereas rabbit anti-STAT3α monoclonal antibody (clone D1A5), also from Cell Signaling Technology (Danvers, MA, USA), was a kind gift from Prof. Cristina Ulivieri (Dept of Life Sciences, University of Siena, Siena, Italy).

Techniques: Expressing, Immunofluorescence, Staining